During the months of January, February and March of this year, police investigated several reports of sexual assault which is believed to have occurred within the TTC system.
Police arrested a man on Thursday following the investigation.
It is alleged that Diego Anesetti of Toronto committed a number of sex-related offences while travelling on the Bloor-Danforth subway line between Yonge/Bloor and Kipling subway stations.
Incidents were also reported to have taken place in the College Park underground shopping area at College and Yonge streets.
Anesetti has been charged with sexual assault, four counts of indecent act, six counts of exposure to a person under 16, mischief interfere with property.
Police believe there may be other victims.